Coding

Part:BBa_K769004

Designed by: Genki Sakai   Group: iGEM12_Tokyo-NoKoGen   (2012-09-19)

NpSRII-9a.a.linker_NpHtrII-EnvZ

This chimeric fusion protein is blue light sensor. This protein is constructed with sensor domain and kinase domain. Sensor domain is sensory rhodopsin II (SRII), which fused by a flexible linker to transducer, halobacterium transducer protein II (HtrII). SRII is a repellent receptor sensing blue light (λ max 487 nm). SRII interacts with HtrII, which in turn controls the activity of a histidine kinase. Histidine kinase we used is the kinase domain of EnvZ. EnvZ phosphorylateｓ response OmpR, which is regulator of two-compoment system. Phosphorylated OmpR activates OmpC promoter.


Safety.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 331
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1027
    Illegal SapI site found at 801


[edit]
Categories
Parameters
None